Proper cell culture media are critical in the maintenance and growth of cell cultures and to allow the reproducibility of experimental results. Some cells additionally need non-essential amino acids (alanine, asparagine, aspartic acid, glutamic acid, glycine, proline, and serine) for effective growth and the reduction of the metabolic burden of ...

First of all when you do long term survival or colony formation assay, you need to keep low concentration of your compound. Secondly, cells should be treated with compound only once of a...

2014年8月5日 · I guess the simplest way is to passage the cells in the compound (i.e. grow in compound to 90% confluence, trypsinise and split into media containing fresh compound).

2023年2月21日 · This review provides a brief introduction about the most common problems encountered in cell culture laboratories and some guidelines on preventing or tackling respective problems.

The aims of the present study were (1) to determine the effectiveness of Plasmocin in eliminating mycoplasmas from infected cultures, (2) to compare Plasmocin with other reagents known to be highly effective, (3) to examine whether any resistance can be overcome by a second round of treatment with Plasmocin or other compounds, (4) to identify ...

2022年11月1日 · Biological contaminants such as bacteria, molds, yeasts, viruses, and mycoplasma as well as cross-contamination by other cell lines form a major part of this problem, and while contamination can never be completely eliminated, proactive measures can help you prevent, detect, and handle cell culture contamination—limiting occurrences and their ...

Developing an understanding of the nature of cells growing in vitro and factors that influence their responsiveness to test compounds will contribute to the development of reproducible cell-based assays.

2022年3月23日 · Cells require careful maintenance to prevent contamination, facilitate growth and ensure long-term stability. The following pages provide general guidance for preserving, thawing and maintaining. Cells in culture can broadly be classified into the following three types. Cells adhere to the culture vessel (e.g. tissue culture plastic).

2023年2月21日 · Cell culture plays an important role in biomedical research. However, cell misidentification, intra- and inter-species cross-contamination, and infection by bacteria, fungi, yeast, or viruses can lead to fatal consequences and pollution of the scientific literature.

Cell culture workflow Use cell lines for standardization and confirmation of each experiment; use primary cells after standardization to further validate the results with a