Scrape each plate of cells into 1 ml of ice-cold EBC lysis buffer (50 mM Tris (pH 8), 120 mM NaCl, 0.5% NP-40, 50 μg/ml leupeptin, 10 μg/ml aprotinin, 50 μg/ml phenylmethylsulfonyl fluoride ...

Mechanical rupture, or lysis, of the cytoplasmic membrane is a common cell death pathway in bacteria occurring in response to β-lactam antibiotics. A better understanding of the cellular design ...

Immunoprecipitates were washed four times with Triton X-100 wash buffer (10 mM Tris-HCl [pH 7.4], 150mM NaCl, 1% Triton X-100), and subjected to SDS-PAGE gel electrophoresis. Equal numbers of HEK293S ...

- max_size (int): Maximum number of experiences the buffer can hold. Defaults to 100,000. The buffer is implemented as a deque (double-ended queue) with a fixed ...

This valuable study reports a chemogenetic screen for resistance and sensitivity to three cell cycle inhibitors used in the clinic: camptothecin, colchicine, and palbociclib. The screen provides a ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. The carboxyl terminus of Hsc70-interacting ...

My choice for the best 40-inch TV is the Samsung QN90D for its updated QLED panel, which provides a brighter picture along with deeper contrast and bolder colors. It also features support for both ...

Evans has demonstrated dynamic leadership as managing director at Zeno Singapore, driving client wins, revenue growth, and championing team welfare and women’s advancement in just eight months.

FD&C Red No. 40, more commonly known as red 40, is making headlines again as lawmakers debate whether food dyes should remain legal in the United States. The dye, which has been registered with ...

You can save this article by registering for free here. Or sign-in if you have an account. Facing the greatest economic threat since at least the 1970s, Prime Minister Justin Trudeau and his would ...