The ability to determine off-target cleavage activity of the CRISPR/Cas9 system is crucial for clinical progression of gene editing. While there has been an influx of off-target sequencing assays ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

DNA sequencing is any chemical, enzymatic or technological procedure for determining the linear order of nucleotide bases in DNA. Sanger sequencing ... analysis of Cas9-induced, unintended large ...

Cell line development plays a pivotal role in the manufacturing of therapeutic proteins, vaccines and other biologics.

At the Nevada Genomics Center we offer DNA sequencing using dye-terminator Sanger sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A Sanger sequencing reaction is run with ...

Honig: Trump is using same theory with federal workers that he used to 'stiff' real estate contractors ...

This study on mouse Ly49 receptors expressed on natural killer (NK) cells shows that Ly49A, in the presence of the corresponding MHC Class I allele, can lead to NK cell licensing, thereby providing ...

Multiple versions of human genomes were created in cell lines, each with different structural changes by researchers from the Wellcome Sanger Institute, Imperial College London, Harvard University in ...

“And rare double stranded breaks may not be detected by existing whole genome sequencing approaches ... “We aim to adapt as new CRISPR systems are developed,” Dobbs said.