A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

Due to the use of dideoxynucleotides in the reactions, Sanger sequencing is also called "dideoxy" sequencing.

Sanger sequencing is the unsung workhorse of molecular biology, one whose value has long been undermined by the tedious nature of processing its data. Converting files of raw sequencing data into ...

This image compares three DNA sequencing technologies: Sanger sequencing, Massively Parallel DNA sequencing, and Nanopore DNA sequencing. Sanger sequencing (left) sequences 500-700 bases per reaction ...

Sanger Sequencing, the workhorse of the human genome project ... PCR products – 5µl PCR product per sequencing reaction (80–100 ng/µl) – sample clean up using Shrimp Alkaline Phosphatase and ...