Scrape each plate of cells into 1 ml of ice-cold EBC lysis buffer (50 mM Tris (pH 8), 120 mM NaCl, 0.5% NP-40, 50 μg/ml leupeptin, 10 μg/ml aprotinin, 50 μg/ml phenylmethylsulfonyl fluoride ...

High-salt wash buffer: 500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8) LiCl wash buffer: 0.25 M LiCl, 1% NP-40 (or IGEPAL ... 300 μl of nuclei lysis buffer by pipetting ...