Reason - this allows the bacteria to spread out and to grow in individual colonies on the agar plate. A lawn of bacteria can be produced by using a sterile spreader to evenly spread the bacteria ...

Sometimes bacteria infect the root but are not able to fix nitrogen. In other situations, bacteria fix nitrogen but cannot enter the root, so nodulation does not occur. Inoculation refers to the ...

Colonies develop sub-surface. 3. Streak plate – the original culture is directly diluted across an agar surface using an inoculating loop. This is a simple & rapid method. All of these methods dilute ...

Farmers growing leguminous crops, the hosts for the nitrogen-fixing rhizobia bacteria, can and should improve nitrogen by inoculating their legume crops with more of the bacteria. Grasslands ...

However, negative staining does not differentiate bacteria, one can only determine morphology. 1. Using a flamed inoculating loop, place 2-3 loopfuls of Congo Red in two separate circles on a clean ...